Müeller-Hinton methylene blue media as an alternative to RPMI 1640 for determining the susceptibility of Cryptococcus neoformans and Cryptococcus gattii to posaconazole with Etest

Mycoses. 2010 Mar 1;53(2):114-6. doi: 10.1111/j.1439-0507.2008.01678.x. Epub 2009 Oct 28.

Abstract

Mueller-Hinton modified agar (MH-GMB) was compared with RPMI + 2% glucose-agar to determine the MICs of 80 isolates of Cryptococcus neoformans and C. gattii to posaconazole with Etest. MH-GMB minimised trailing and agreement between both media was 94%. Agreement of M27-A2 microbroth reference method was 98% with RPMI and 94% with MB-GMB.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Antifungal Agents / pharmacology*
  • Cryptococcus gattii / drug effects*
  • Cryptococcus neoformans / drug effects*
  • Culture Media / chemistry*
  • Humans
  • Microbial Sensitivity Tests / methods
  • Triazoles / pharmacology*

Substances

  • Antifungal Agents
  • Culture Media
  • Triazoles
  • posaconazole