Genetic characterization of G3 rotaviruses detected in Italian children in the years 1993-2005

J Med Virol. 2009 Dec;81(12):2089-95. doi: 10.1002/jmv.21615.

Abstract

In recent years an apparent increase in the frequency of detection of G3P[8] rotaviruses has been observed worldwide. Similarly, in Italy G3P[8] strains have been detected sporadically and in a scattered fashion over 20 years, whereas in 2003 and 2005 G3P[8] rotavirus activity increased markedly. By analysis of the VP7, VP4, VP6, and NSP4 genes of a selection of G3P[8] rotaviruses detected between 1993 and 2005, a remarkable sequence conservation was observed in the VP7, VP4, and VP6 genes. By converse, after 2002 the Italian G3P[8] strains were found to possess unique mutations in significant regions of the NSP4 protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / analysis
  • Antigens, Viral / genetics
  • Capsid Proteins / analysis
  • Capsid Proteins / genetics
  • Child, Preschool
  • Feces / virology
  • Glycoproteins / analysis
  • Glycoproteins / genetics
  • Humans
  • Infant
  • Infant, Newborn
  • Italy / epidemiology
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Rotavirus / genetics*
  • Rotavirus Infections* / epidemiology
  • Rotavirus Infections* / virology
  • Sequence Analysis, RNA
  • Toxins, Biological / analysis
  • Toxins, Biological / genetics
  • Viral Nonstructural Proteins / analysis
  • Viral Nonstructural Proteins / genetics

Substances

  • Antigens, Viral
  • Capsid Proteins
  • Glycoproteins
  • NS28 protein, rotavirus
  • RNA, Viral
  • Toxins, Biological
  • VP4 protein, Rotavirus
  • VP6 protein, Rotavirus
  • VP7 protein, Rotavirus
  • Viral Nonstructural Proteins