Synthesis of new heterometallic macromolecules: their DNA binding, cleavage activity and in vitro model electrochemotherapy study

Spectrochim Acta A Mol Biomol Spectrosc. 2009 Dec;74(5):1152-9. doi: 10.1016/j.saa.2009.09.025. Epub 2009 Sep 18.

Abstract

The homodinuclear C(16)H(30)N(8)O(5)Sn(2)Cl(4) (1), heterotetranuclear C(16)H(38)N(8)O(9)Sn(2)Cu(2)Cl(8) (2) and C(16)H(38)N(8)O(9)Sn(2)Mn(2)Cl(8) (3) macrocyclic complexes were synthesized and characterized by elemental analysis, spectroscopic techniques and molar conductance measurements. The interaction studies of 1-3 with calf thymus DNA (CT-DNA) were carried out by UV-vis titration, fluorescence, cyclic voltammetry and viscosity measurements. These results were further authenticated by carrying out interaction studies of 1-3 with plasmid pBR322 DNA employing gel electrophoresis. To overcome the dose resistance, auto toxicity of the drugs, a model study based on electrochemotherapy (ECT) was carried out and the results were compared in the presence and in the absence of the applied electrical potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Animals
  • Cattle
  • DNA / metabolism*
  • Drug Therapy / methods*
  • Electrochemistry / methods*
  • Electron Spin Resonance Spectroscopy
  • Electrons
  • Fluorescence
  • Macrocyclic Compounds / chemical synthesis*
  • Macrocyclic Compounds / chemistry
  • Macrocyclic Compounds / metabolism*
  • Magnetic Resonance Spectroscopy
  • Metals
  • Models, Chemical
  • Spectrophotometry, Infrared
  • Viscosity

Substances

  • Macrocyclic Compounds
  • Metals
  • DNA
  • calf thymus DNA