Abstract
A single substitution within the hepatitis C virus core antigen sequence, A48T, which is observed in approximately 30% of individuals infected with genotype 2a virus, reduces the sensitivity of a commonly used chemiluminescence enzyme immunoassay. Quantitation of the antigen is improved by using a distinct anticore antibody with a different epitope.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution
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Enzyme-Linked Immunosorbent Assay
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Genotype
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Hepacivirus* / classification
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Hepacivirus* / genetics
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Hepatitis C / diagnosis*
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Hepatitis C / virology
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Hepatitis C Antigens / analysis*
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Hepatitis C Antigens / chemistry
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Hepatitis C Antigens / genetics
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Humans
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Immunoblotting
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Immunoenzyme Techniques
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Molecular Sequence Data
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Recombinant Proteins / analysis*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Viral Core Proteins / analysis*
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Viral Core Proteins / chemistry
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Viral Core Proteins / genetics
Substances
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Hepatitis C Antigens
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Recombinant Proteins
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Viral Core Proteins