Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: effect of organic solvent and initial water content

Takanori Tanino; Takumi Ohno; Chiaki Ogino; Hideki Fukuda; Akihiko Kondo

doi:10.1016/j.jbiosc.2009.05.006

Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: effect of organic solvent and initial water content

J Biosci Bioeng. 2009 Nov;108(5):369-71. doi: 10.1016/j.jbiosc.2009.05.006.

Authors

Takanori Tanino¹, Takumi Ohno, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

Affiliation

¹ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.

PMID: 19804858
DOI: 10.1016/j.jbiosc.2009.05.006

Abstract

The effect of the reaction conditions on the ester synthesis reaction with CALB displaying yeast whole cells was determined. Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the ester synthesis activity of CALB displaying yeast whole cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Cell Culture Techniques / methods
Esterification
Esters / metabolism
Fungal Proteins
Lipase / metabolism*
Organic Chemicals / metabolism*
Solvents / metabolism
Water / metabolism*

Substances

Esters
Fungal Proteins
Organic Chemicals
Solvents
Water
Lipase
lipase B, Candida antarctica