A two-year field located monitoring was carried out to study the impacts of hydro-periods on the lakeshore meadow wetland soil total nitrogen (TN) and hydrolysable nitrogen (HN) in Napahai plateau wetland in northwestern Yunnan province. The results show that meadow soil alternate drying-wetting is the significant characteristic influenced by hydro-periods. Hydro-periods of meadow wetland control the physical environment of meadow soil, and the occurrence of TN in the upper layer (0-20 cm) was influenced more significantly than in the deeper layer (20-40 cm) by wetland hydro-periods. The dynamics of TN (0.96-1.30 g/kg) show a shape of anomalous "W" in the upper layer. And in the rainy season, the dynamics of TN was the same as the trend of water level fluctuation, while the HN was in the reverse trend. The enzyme activities were influenced by hydro-periods and then controlled the mineralization process of organic nitrogen. In August when wetland water fell, the highest amount of HN was 222.19 mg/kg (0-20 cm layer) and 47.41 mg/kg (20-40 cm layer) respectively. But when the wetland water level rose, the HN was moved to wetland water by the rising water level, and the decrease amplitude was 42% and 48% respectively. Therefore the deterioration of the environment of water was aggravated.