Cellulase assays

Methods Mol Biol. 2009:581:213-31. doi: 10.1007/978-1-60761-214-8_14.

Abstract

Cellulose is a heterogeneous polysaccharide, and its enzymatic hydrolysis requires endoglucanase, exoglucanase (cellobiohydrolase), and beta-glucosidase to work together. We summarize the most commonly used assays for individual enzymes and cellulase mixture.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Anaerobiosis / physiology
  • Cellulase / analysis
  • Cellulase / metabolism*
  • Cellulose / metabolism
  • Disaccharides / metabolism
  • Electrophoresis / methods
  • Enzyme Assays / methods*
  • Hydrolysis
  • beta-Glucosidase / metabolism

Substances

  • Disaccharides
  • Cellulose
  • beta-Glucosidase
  • Cellulase