At the microvascular level, the radial oxygen gradient is greater in arterioles than in any other vascular segment and thus drives the oxygen from the blood (high concentration, source) into the perivascular tissue (low concentration, sink). Thus, arterioles appear to be the main suppliers of oxygen to the tissue, in contrast to the capillaries, where the oxygen gradient is only a few millimeters of mercury. However, longitudinal oxygen loss from arteriolar blood is higher than can be solely accounted for by diffusion. This discrepancy becomes evident when determining how oxygen is distributed in the microvascular network, an approach that requires confirmation of the data in terms of mass balance and thermodynamic considerations. A fundamental difficulty is that measuring tissue P