Development of a rapid and reversible photocrosslinking of RNA

Yoshinaga Yoshimura; Tomoko Ohtake; Hajime Okada; Kenzo Fujimoto

doi:10.1093/nass/nrp099

Development of a rapid and reversible photocrosslinking of RNA

Nucleic Acids Symp Ser (Oxf). 2009:(53):197-8. doi: 10.1093/nass/nrp099.

Authors

Yoshinaga Yoshimura¹, Tomoko Ohtake, Hajime Okada, Kenzo Fujimoto

Affiliation

¹ School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.

PMID: 19749328
DOI: 10.1093/nass/nrp099

Abstract

We describe a novel reversible interstrand photocrosslinking reaction of RNA. In this system, a modified oligodeoxynucleotide (ODN) containing 3-cyanovinylcarbazole nucleoside ((CNV)K) reacts by photoirradiation at 366 nm for 1 s with pyrimidine residue of a complementary template RNA to yield a crosslinked product in 94% yield.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbazoles / chemistry
DNA / chemistry
Oligodeoxyribonucleotides / chemistry
Oligodeoxyribonucleotides / radiation effects
Photochemical Processes
RNA / chemistry*
RNA / radiation effects
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Carbazoles
Oligodeoxyribonucleotides
RNA
DNA