Abstract
A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid and simultaneous typing and subtyping of influenza viruses.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzothiazoles
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Diamines
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Humans
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Influenza A Virus, H1N1 Subtype / classification*
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / isolation & purification*
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Influenza, Human / virology*
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Oligonucleotide Probes / genetics
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Organic Chemicals / metabolism
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Quinolines
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Staining and Labeling / methods
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Time Factors
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Viral Proteins / genetics
Substances
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Benzothiazoles
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Diamines
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Oligonucleotide Probes
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Organic Chemicals
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Quinolines
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Viral Proteins
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SYBR Green I