Rapid SYBR green I and modified probe real-time reverse transcription-PCR assays identify influenza H1N1 viruses and distinguish between pandemic and seasonal strains

J Clin Microbiol. 2009 Nov;47(11):3714-6. doi: 10.1128/JCM.01646-09. Epub 2009 Sep 9.

Abstract

A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid and simultaneous typing and subtyping of influenza viruses.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles
  • Diamines
  • Humans
  • Influenza A Virus, H1N1 Subtype / classification*
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / isolation & purification*
  • Influenza, Human / virology*
  • Oligonucleotide Probes / genetics
  • Organic Chemicals / metabolism
  • Quinolines
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staining and Labeling / methods
  • Time Factors
  • Viral Proteins / genetics

Substances

  • Benzothiazoles
  • Diamines
  • Oligonucleotide Probes
  • Organic Chemicals
  • Quinolines
  • Viral Proteins
  • SYBR Green I