The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis

Comp Immunol Microbiol Infect Dis. 2010 Dec;33(6):485-9. doi: 10.1016/j.cimid.2009.08.003. Epub 2009 Sep 6.

Abstract

In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p<0.01) between reactivity of sera from these groups, encouraging the study of purified proteins to differentiate between both diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / immunology*
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / immunology
  • Diagnosis, Differential
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Membrane Proteins / immunology*
  • Paratuberculosis / diagnosis*
  • Paratuberculosis / immunology
  • Sensitivity and Specificity
  • Tuberculosis, Bovine / diagnosis*
  • Tuberculosis, Bovine / immunology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • MPB83 protein, Mycobacterium
  • Membrane Proteins
  • MPB70 protein, Mycobacterium bovis