Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes

J Biomed Opt. 2009 Jul-Aug;14(4):040506. doi: 10.1117/1.3207151.

Abstract

We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Chickens
  • Equipment Design
  • Equipment Failure Analysis
  • Erythrocytes / metabolism*
  • Hemoglobins / analysis*
  • Mice
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staining and Labeling
  • Tissue Distribution

Substances

  • Hemoglobins