Abstract
Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.
(c) 2009 by John Wiley & Sons, Inc.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Biological Assay
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Cell Culture Techniques / methods*
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Cell Differentiation*
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Cell Lineage*
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Cell Proliferation
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Cell Separation
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Cell Survival
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Cryopreservation
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Humans
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Myelin Sheath / metabolism
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Neural Cell Adhesion Molecules / metabolism
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Neurons / cytology
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Oligodendroglia / cytology*
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Rats
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Stem Cell Transplantation
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Stem Cells / cytology
Substances
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Neural Cell Adhesion Molecules