It is well known that there is a lack of psychometric studies testing sexual function in drug-dependent population. Thus, the main objective of this work was to carry out the first psychometric analysis of the Brief Sexual Functioning Inventory (BSFI) in a sample of drug users (N = 104). Participants filled out the BSFI, Changes in Sexual Functioning Questionnaire (CSFQ-14) and Addictive Behavior Questionnaire (ABQ). The BSFI shows a five-factor structure similar to the original (Sexual drive, alpha = .75; Satisfaction, alpha = .63; Erectile function, alpha = .70; Ejaculatory function, alpha = .74; and Problem Assessment, alpha = .70), though compared to the original scale two items are loading in a different dimension. The high and significant correlations with the CSFQ-14 indicated adequate validity of the measures. Capacity of the BSFI to discriminate between clinical and non-clinical samples is discussed.