Synthesis of 13C7-labeled iodoacetanilide and application to quantitative analysis of peptides and a protein by isotope differential mass spectrometry

Bioorg Med Chem Lett. 2009 Oct 1;19(19):5698-702. doi: 10.1016/j.bmcl.2009.08.011. Epub 2009 Aug 7.

Abstract

(13)C(7)-Labeled iodoacetanilide has been synthesized for specific labeling of sulfhydryl groups of cysteine residues and has been successfully applied to quantitative analysis of peptides and a commercial protein in combination with (13)C-unlabeled iodoacetanilide and a MALDI mass spectrometer. Subsequent tandem mass spectrum analysis revealed that (13)C(7)-labeled iodoacetanilide remained intact during the collision-induced dissociation (CID) conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetanilides / chemical synthesis*
  • Acetanilides / chemistry
  • Animals
  • Carbon Isotopes / chemistry
  • Cattle
  • Isotope Labeling
  • Peptides / analysis*
  • Proteins / analysis*
  • Serum Albumin, Bovine / analysis
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Acetanilides
  • Carbon Isotopes
  • Peptides
  • Proteins
  • iodoacetanilide
  • Serum Albumin, Bovine