Quantitative trait loci (QTLs) for chlorophyll content were studied using a doubled haploid (DH) population with 168 progeny lines, derived from a cross between two elite Chinese wheat cultivars Huapei 3 x Yumai 57. Chlorophyll content was evaluated at the maximum tillering stage (MS), the heading stage (HS), and the grain filling stage (GS), at three different environments in 2005 and 2006 cropping seasons. QTL analyses were performed using a mixed linear model approach. A total of 17 additive QTLs and nine pairs of epistatic QTLs were detected. Ten of 17 additive QTLs for chlorophyll content were persistently expressed at more than two growth stages, which suggest developmentally regulated loci controlling genetics for chlorophyll content in different growth stages in wheat. One novel major QTL for chlorophyll content was closely linked with the PCR marker Xwmc215 and was persistently expressed at three growth stages.