Objective: To study the protective mechanism of complex vitamin B (Co VB), GSH and both application on the rat fatty liver.
Methods: 50 male Wistar rats were allocated to 5 groups randomly, which were normal control group (NC), model control group (TC), intervention of Co VB (T-VB), intervention of GSH (T-glu), intervention of Co VB and GSH (T-glu+ B), respectively. Except NC, other groups were treated with a low dose of Carbon tetrachloride (CCl4), high fat diet and alcohol water solution to produce fatty liver model. At the same time, experimental groups were drenched Co VB or (and) GSH, respectively. NC and TC group was drenched physiological salt solution for 28 days. The serum and liver lipid were quantified. The content of MDA and activity of SOD in liver were measured, and histomorphology changes of the liver were observed, and so on.
Results: Compared with NC group, rat hepatic cells showed adipose degeneration, and the content of liver MDA and serum ALT was increased in TC group. The content of both serum and hepatic tissue TC, TG were increased, the content of serum AST and HDL were increased, the activity of liver SOD was decreased vs. NC group (P < 0.05). After intervention, adipose degeneration of experiment groups hepatic cells was obviously inhibited, the content of both serum and (or) hepatic tissue TC, TG, ALT and MDA were decreased (P < 0.05), but the content of serum HDL and the activity of liver SOD were increased vs. TC group(P < 0.05).
Conclusion: Co VB and GSH have the protection on fatty liver of rats, the mechanism is that they can inhibit lipid metabolic disorder and antioxidative damage.