Quercetin-3-O-beta-d-glucuronopyranoside (QGC)-induced HO-1 expression through ERK and PI3K activation in cultured feline esophageal epithelial cells

Fitoterapia. 2010 Mar;81(2):85-92. doi: 10.1016/j.fitote.2009.08.008. Epub 2009 Aug 15.

Abstract

Heme oxygenase-1 (HO-1) is one of the antioxidant enzymes which help protect against cellular damage. The present study examined the ability of Quercetin-3-O-beta-D-glucuronopyranoside (QGC), flavonoid glucoside extracted from Rumex Aquaticus Herba, to induce expression of HO-1 and analyzed its signaling mechanism in cultured feline esophageal epithelial cells (EEC). Culture of the esophageal epithelial cells from cat was prepared. The data suggested that QGC could result in enhanced antioxidant enzyme defense system via HO-1 expression and Nrf2 translocation involving both the ERK and PI3K-Akt pathways as well as partly PKC pathways in EEC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Cats
  • Cells, Cultured
  • Epithelial Cells / drug effects*
  • Epithelial Cells / metabolism
  • Esophagus / drug effects
  • Esophagus / metabolism
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Glycosides / pharmacology*
  • Heme Oxygenase-1 / biosynthesis*
  • NF-E2-Related Factor 2 / metabolism
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Protein Kinase C / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism
  • Quercetin / analogs & derivatives*
  • Quercetin / pharmacology
  • Rumex / chemistry*
  • Signal Transduction

Substances

  • Antioxidants
  • Glycosides
  • NF-E2-Related Factor 2
  • Plant Extracts
  • quercetin 3-O-glucuronide
  • Quercetin
  • Heme Oxygenase-1
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins c-akt
  • Protein Kinase C
  • Extracellular Signal-Regulated MAP Kinases