Discovery of small peptides derived from embryonic lethal abnormal vision proteins structure showing RNA-stabilizing properties

J Med Chem. 2009 Aug 27;52(16):5017-9. doi: 10.1021/jm900741e.

Abstract

Four peptides corresponding to highly conserved motives within the first two RNA recognition motif-type domains of ELAV proteins were prepared, and their effect on the stability of NOVA-1 and VEGF ELAV-target mRNAs was evaluated. Biological results show that in the presence of phorbol 12-myristate 13-acetate (a PKC activator triggering the ELAV pathway), an equimolar mixture of peptides induces a statistically significant stabilization of the selected transcripts, suggesting a synergic effect of the two stimuli.

MeSH terms

  • Amino Acid Motifs
  • Antigens, Neoplasm / genetics*
  • Cell Line, Tumor
  • Conserved Sequence
  • ELAV Proteins / chemistry*
  • Enzyme Activators / pharmacology
  • Humans
  • Nerve Tissue Proteins / genetics*
  • Neuro-Oncological Ventral Antigen
  • Oligopeptides / chemical synthesis*
  • Oligopeptides / chemistry
  • Oligopeptides / pharmacology
  • Protein Kinase C / metabolism
  • RNA Stability / drug effects*
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Vascular Endothelial Growth Factor A / genetics*

Substances

  • Antigens, Neoplasm
  • ELAV Proteins
  • Enzyme Activators
  • Nerve Tissue Proteins
  • Neuro-Oncological Ventral Antigen
  • Oligopeptides
  • RNA, Messenger
  • RNA-Binding Proteins
  • Vascular Endothelial Growth Factor A
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate