[Methods for detection of methylated cytosine residues in DNA]

Mol Biol (Mosk). 2009 May-Jun;43(3):387-91.
[Article in Russian]

Abstract

The article provides analysis of common methods for DNA methylation detection. Advantages and limitations of methods used for different purposes are compared. Clue step for most common methods is bisulfite treatment of DNA samples and its protocol is described in details. Recommendations are formulated for each method best in solving specific problems.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cytosine / chemistry
  • Cytosine / metabolism*
  • DNA / chemistry
  • DNA / metabolism*
  • DNA Methylation*
  • Indicators and Reagents
  • Nucleic Acid Denaturation
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA / methods
  • Sulfites / chemistry

Substances

  • Indicators and Reagents
  • Sulfites
  • Cytosine
  • DNA
  • sodium bisulfite