Objective: To study the effect of shRNA-CXCR4 on human breast cancer cells migration and invasion.
Methods: Through knockdown CXCR4 by shRNA, the CXCR4 mRNA expression and protein expression were examined with RT-PCR and Western blot separately. The matrigel penetrating ability of human breast cancer cells was examined in vitro experiments.
Results: The CXCR4 mRNA express and its protein express decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells (P<0.05). The invasive ability of human breast cancer cells was significantly decreased (P<0.05).
Conclusion: through knockdown CXCR4 by shRNA, the invasive ability of human breast cancer cells can be inhibited obviously.