[Preparation and characterization of monoclonal antibodies to the cholera toxin]

E E Petrova; R L Komaleva; O E Lakhtina; L V Samokhvalova; N A Kalinina; N S Shoshina; A Iu Rubina; M A Filippova; Iu V Vertiev; T I Valyakina; E V Grishin

doi:10.1134/s1068162009030078

[Preparation and characterization of monoclonal antibodies to the cholera toxin]

Bioorg Khim. 2009 May-Jun;35(3):357-67. doi: 10.1134/s1068162009030078.

[Article in Russian]

Authors

E E Petrova¹, R L Komaleva, O E Lakhtina, L V Samokhvalova, N A Kalinina, N S Shoshina, A Iu Rubina, M A Filippova, Iu V Vertiev, T I Valyakina, E V Grishin

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho-Maklaya 16/10, V-437, Moscow, 117997 Russia.

PMID: 19621051
DOI: 10.1134/s1068162009030078

Abstract

Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Bacterial / immunology*
Antibodies, Monoclonal / immunology*
Antibodies, Monoclonal / isolation & purification
Bacterial Toxins / immunology
Cholera Toxin / immunology*
Cross Reactions
Escherichia coli Proteins / immunology
Immunoenzyme Techniques
Microarray Analysis

Substances

Antibodies, Bacterial
Antibodies, Monoclonal
Bacterial Toxins
Escherichia coli Proteins
Cholera Toxin