Electrospinning is a new method used in tissue engineering. It can spin fibers in nanoscale by electrostatic force. A series of thermoplastic polyurethane (TPU)/collagen blend nanofibrous membranes was prepared with different weight ratios and concentrations via electrospinning. The two biopolymers used 1,1,1,3,3,3,-hexafluoro-2-propanol (HFP) as solvent. The electrospun TPU-contained collagen nanofibers were characterized using scanning electron microscopy (SEM), XPS spectroscopy, atomic force microscopy, apparent density and porosity measurement, contact-angle measurement, mechanical tensile testing and viability of pig iliac endothelial cells (PIECs) on blended nanofiber mats. Our data indicate that fiber diameter was influenced by both polymer concentration and blend weight ratio of collagen to TPU. The average diameter of nanofibers gradually decreases with increasing collagen content in the blend. XPS analysis indicates that collagen is found to be present at the surface of blended nanofiber. The results of porosity and contact-angle measurement suggest that with the collagen content in the blend system, the porosity and hydrophilicity of the nanofiber mats is greatly improved. We have also characterized the molecular interactions in TPU/collagen complex by Fourier transform infrared spectroscopy (FT-IR). The result could demonstrate that there were no intermolecular bonds between the molecules of TPU and collagen. The ultimate tensile stress and strain were carried out and the data confirmed the FT-IR results. The TPU/collagen blend nanofibrous mats were further investigated as promising scaffold for PIEC culture. The cell proliferation and SEM morphology observations showed that the cells could not only favorably grow well on the surface of blend nanofibrous mats, but also able to migrate inside the scaffold within 24 h of culture. These results suggest that the blend nanofibers of TPU/collagen are designed to mimic the native extracellular matrix for tissue engineering and develop functional biomaterials.
(c) Koninklijke Brill NV, Leiden, 2009