Quantitative measurement of pH influence on SalR regulated gene expression in Acinetobacter baylyi ADP1

Chao Li; Hui Wang; Lin Zhou; Yongjun Zhang; Fuping Song; Jie Zhang

doi:10.1016/j.mimet.2009.07.004

Quantitative measurement of pH influence on SalR regulated gene expression in Acinetobacter baylyi ADP1

J Microbiol Methods. 2009 Oct;79(1):8-12. doi: 10.1016/j.mimet.2009.07.004. Epub 2009 Jul 17.

Authors

Chao Li¹, Hui Wang, Lin Zhou, Yongjun Zhang, Fuping Song, Jie Zhang

Affiliation

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No.2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.

PMID: 19616585
DOI: 10.1016/j.mimet.2009.07.004

Abstract

The influence of medium pH conditions on regulation of the sal operon was quantitatively measured by using a genetically engineered Acinetobacter baylyi ADP1 strain ADPWH_lux. Live-cell based bioluminescence assays determined that the SalR, a LysR-type transcriptional regulator (LTTR), functioned in a range of medium pH conditions. During exponential growth, SalR regulated bioluminescence production peaked at the neutral pH and tolerated medium pH changes of 5.5-9.0. In the stationary phase, the SalR regulated gene expression reached 20-35% of the maximum productivity during the exponential phase. This report reveals the influence of pH conditions on a LTTR system and demonstrates that the biosensor strain can be a useful tool for understanding transcriptional regulations in different environments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter / drug effects*
Acinetobacter / physiology*
Artificial Gene Fusion
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Genes, Reporter
Hydrogen-Ion Concentration
Luminescence
Luminescent Proteins
Operon
Stress, Physiological*
Transcription Factors / metabolism*

Substances

Luminescent Proteins
Transcription Factors