Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt 7):730-2. doi: 10.1107/S1744309109023392. Epub 2009 Jun 30.

Abstract

The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoproteins / chemistry*
  • Bacterial Proteins / chemistry*
  • Crystallization
  • Crystallography, X-Ray
  • Desulfovibrio gigas / chemistry*
  • Electrophoresis, Polyacrylamide Gel

Substances

  • Apoproteins
  • Bacterial Proteins