Abstract
Interferon-beta (IFN-beta), acting canonically via the modulation of transcription, affects neocortical pyramidal neurons. By use of 2-D differential gel electrophoresis and subsequent mass spectrometry we identified IFN-beta regulated proteins in the central nervous system. These proteins are involved in cytoskeleton assembly, protein transport and nucleotide metabolism and, as such, serve regenerative and protective functions. Electrophysiologically, IFN-beta mediated protein synthesis is essential for part of the excitatory neuronal effects, as revealed under blockade of protein biosynthesis. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation / physiology
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Central Nervous System / cytology
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Central Nervous System / drug effects*
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Central Nervous System / metabolism
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Cerebral Cortex / cytology
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism
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Cycloheximide / pharmacology
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Cytoskeleton / drug effects
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Cytoskeleton / metabolism
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Electrophoresis, Gel, Two-Dimensional
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Electrophysiology
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Interferon-beta / metabolism
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Interferon-beta / pharmacology*
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Male
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Mass Spectrometry
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Membrane Potentials
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / drug effects*
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Neurogenesis / drug effects
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Neurogenesis / physiology
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Neurons / drug effects*
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Neurons / metabolism
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Neurons / ultrastructure
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Organ Culture Techniques
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Protein Synthesis Inhibitors / pharmacology
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Proteome / analysis
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Proteome / drug effects*
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Proteome / metabolism
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Proteomics / methods
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Pyramidal Cells / drug effects
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Pyramidal Cells / metabolism
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Rats
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Rats, Wistar
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Up-Regulation / drug effects
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Up-Regulation / physiology
Substances
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Nerve Tissue Proteins
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Protein Synthesis Inhibitors
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Proteome
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Interferon-beta
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Cycloheximide