A study of inclusion complexation of liquid non-ionic surfactants, nonylphenol (NP) and nonylphenol 9 mole ethoxylate (NP9EO), with beta-cyclodextrin (beta-CD), was carried out by mass spectrometry, surface tension, and ultraviolet-visible (UV-VIS) and Fourier transform infrared (FTIR) spectroscopies. The inclusion complexation was effectuated by heating at 80 degrees C and filtration of aqueous NP+beta-CD and NP9EO+beta-CD suspensions. The mass spectrometry and surface tension measurements revealed that NP and NP9EO form inclusion complexes with beta-CD and beta-CD possesses a higher affinity for NP. These results are supported by the data from UV-VIS spectroscopic analyses that have indicated that a three times greater amount of NP is entrapped into beta-CD than NP9EO. This phenomenon has been associated with the smaller size and a higher degree of hydrophobicity of NP that favours its entrapment into beta-CD as compared to that of NP9EO. At the structural level, the data from FTIR spectroscopic study have indicated that alkyl chains of NP and NP9EO can form van der Waals interactions with the cavity of beta-CD. Moreover, NP and NP9EO seem to cause a reorganization of the intramolecular hydrogen bonds and change of the hydration of beta-CD, but did not appear to strongly interact with C-C, C-O-C, and OH groups of beta-CD. Together these results suggest that the formation of inclusion complexes by NP and NP9EO with beta-CD molecules could constitute an effective and advantageous technique to remove liquid non-ionic surfactants from wastewater due to the non-toxic character of beta-CD to humans and the environment.