Tetra primer ARMS-PCR for identification of SNP in beta-tubulin of Botrytis cinerea, responsible of resistance to benzimidazole

J Microbiol Methods. 2009 Aug;78(2):245-6. doi: 10.1016/j.mimet.2009.06.007. Epub 2009 Jun 17.

Abstract

A competitive polymerase chain reaction (PCR) technique called Tetra primer amplification refractory mutation system (ARMS) PCR was adapted to identify a single nucleotide polymorphism (SNP) in beta-tubulin gene of Botrytis cinerea. Over the 35 isolates analyzed, six of them showed a SNP in that gene, which was readily identified by the technique and in the six cases correspond to the resistant strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Benzimidazoles / pharmacology*
  • Botrytis / drug effects
  • Botrytis / genetics*
  • DNA Primers / genetics
  • DNA, Fungal / genetics
  • Drug Resistance, Fungal*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Single Nucleotide*
  • Tubulin / genetics*

Substances

  • Antifungal Agents
  • Benzimidazoles
  • DNA Primers
  • DNA, Fungal
  • Tubulin
  • benzimidazole