Abstract
A competitive polymerase chain reaction (PCR) technique called Tetra primer amplification refractory mutation system (ARMS) PCR was adapted to identify a single nucleotide polymorphism (SNP) in beta-tubulin gene of Botrytis cinerea. Over the 35 isolates analyzed, six of them showed a SNP in that gene, which was readily identified by the technique and in the six cases correspond to the resistant strain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antifungal Agents / pharmacology*
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Benzimidazoles / pharmacology*
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Botrytis / drug effects
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Botrytis / genetics*
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DNA Primers / genetics
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DNA, Fungal / genetics
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Drug Resistance, Fungal*
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Polymerase Chain Reaction / methods*
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Polymorphism, Single Nucleotide*
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Tubulin / genetics*
Substances
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Antifungal Agents
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Benzimidazoles
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DNA Primers
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DNA, Fungal
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Tubulin
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benzimidazole