Structured illumination microscopy of a living cell

Eur Biophys J. 2009 Jul;38(6):807-12. doi: 10.1007/s00249-009-0501-6. Epub 2009 Jun 18.

Abstract

Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Image Processing, Computer-Assisted / methods
  • Light*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Microspheres
  • Mitochondria / physiology*
  • Mitochondria / ultrastructure*
  • Motion
  • Polystyrenes
  • Time Factors

Substances

  • Polystyrenes