Abstract
GW182 family proteins interact directly with Argonaute proteins and are required for miRNA-mediated gene silencing in animal cells. The domains of the GW182 proteins have recently been studied to determine their role in silencing. These studies revealed that the middle and C-terminal regions function as an autonomous domain with a repressive function that is independent of both the interaction with Argonaute proteins and of P-body localization. Such findings reinforce the idea that GW182 proteins are key components of miRNA repressor complexes in metazoa.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Amino Acid Sequence
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Animals
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Autoantigens / chemistry*
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Autoantigens / genetics
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Autoantigens / metabolism*
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Binding Sites / genetics
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Caenorhabditis elegans Proteins / chemistry
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism
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Conserved Sequence
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Drosophila Proteins / chemistry
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism
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Eukaryotic Initiation Factors / metabolism
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Gene Silencing*
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Humans
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MicroRNAs / genetics*
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MicroRNAs / metabolism*
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Models, Genetic
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Models, Molecular
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Molecular Sequence Data
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Protein Structure, Tertiary
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RNA Processing, Post-Transcriptional
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RNA-Binding Proteins
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Sequence Homology, Amino Acid
Substances
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Autoantigens
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Caenorhabditis elegans Proteins
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Drosophila Proteins
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Eukaryotic Initiation Factors
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Gw protein, Drosophila
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MicroRNAs
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RNA-Binding Proteins
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TNRC6A protein, human