Abstract
The widely used hepatitis B virus (HBV) vaccine is based on three doses of hepatitis B surface antigen (HBsAg) protein. We previously showed that vectored measles viruses (MV) expressing HBsAg retain measles vaccine function in monkeys but do not induce a protective anti-HBs response in all animals. We show here that a single dose of HBsAg protein following a three-dose vaccination regimen with an optimized HBsAg-expressing MV elicits protective anti-HBs responses in all four vaccinated Rhesus monkeys. Vaccination strategies coupling the effective, long-term immunity elicited by the high-coverage MV vaccine to prophylactic HBV immunity are discussed.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Genetic Vectors*
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Hepatitis B / prevention & control*
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Hepatitis B Antibodies / blood
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Hepatitis B Surface Antigens / genetics
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Hepatitis B Surface Antigens / immunology*
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Hepatitis B Vaccines / genetics
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Hepatitis B Vaccines / immunology*
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Humans
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Immunization / methods*
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Interferon-gamma / metabolism
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Leukocytes, Mononuclear / immunology
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Macaca mulatta
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Measles virus / genetics*
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Neutralization Tests
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Vaccines, Subunit / genetics
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Vaccines, Subunit / immunology
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Viremia / prevention & control
Substances
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Hepatitis B Antibodies
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Hepatitis B Surface Antigens
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Hepatitis B Vaccines
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Vaccines, Subunit
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Vaccines, Synthetic
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Interferon-gamma