Paramagnetic labelling of proteins and oligonucleotides for NMR

J Biomol NMR. 2010 Jan;46(1):101-12. doi: 10.1007/s10858-009-9331-1. Epub 2009 Jun 16.

Abstract

Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Electron Spin Resonance Spectroscopy / methods*
  • Lanthanoid Series Elements / chemistry
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Oligonucleotides / chemistry*
  • Protein Conformation*
  • Proteins / chemistry*
  • Spin Labels

Substances

  • Lanthanoid Series Elements
  • Oligonucleotides
  • Proteins
  • Spin Labels