The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3' ends in a plasmid-only-based virus rescue system

Arch Virol. 2009;154(7):1151-6. doi: 10.1007/s00705-009-0422-9. Epub 2009 Jun 13.

Abstract

Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Animals
  • Chlorocebus aethiops
  • DNA-Directed RNA Polymerases / genetics
  • Genes, Reporter
  • Genome, Viral
  • Green Fluorescent Proteins / genetics
  • Hepatitis Delta Virus / genetics*
  • Humans
  • Mice
  • Plasmids
  • Promoter Regions, Genetic
  • RNA Polymerase I / genetics*
  • RNA, Catalytic / genetics
  • RNA, Viral / genetics
  • Restriction Mapping
  • Terminator Regions, Genetic
  • Transcription, Genetic
  • Vero Cells
  • Virus Replication

Substances

  • 3' Untranslated Regions
  • RNA, Catalytic
  • RNA, Viral
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • DNA-Directed RNA Polymerases
  • RNA Polymerase I