The microbial behaviour of five enterotoxigenic strains of Staphylococcus aureus was studied in the growth/no growth domain. A polynomial logistic regression equation was fitted using a stepwise method to study the interaction of temperature (8, 10, 13, 16 and 19 degrees C), pH (4.5; 5.0; 5.5; 6.0; 6.5 7.0 and 7.5) and water activity (A(w)) (19 levels ranging from 0.867 to 0.999) on the probability of growth. Out of the 284 conditions tested, 146 were chosen for model data and 138 intermediate conditions for validation data. A growth/no growth transition was obtained by increasing the number of replicates per condition (n=30) in comparison to other published studies. The logistic regression model showed a good performance since 96.6% (141 out of 146 conditions) of the conditions for model data and 92.0% (127 out of 138 conditions) for validation data were correctly classified. The predictions indicated an abrupt growth/no growth interfaces occurred at low levels of temperature, pH and A(w). At 8 degrees C, S. aureus grew only at optimum levels of pH and A(w) while at temperatures above 13 degrees C, growth of S. aureus was observed at pH=4.5 and A(w)=0.96 (13 degrees C), 0.941 (16 degrees C) and 0.915 (19 degrees C). The optimal pH at which growth of S. aureus was detected earlier was 6.5. However, a slight decrease of the probability of growth was noticed in the pH interval of 7.0-7.5 at more stringent conditions. The ability of S. aureus to grow at low A(w) was shown since growth was detected at A(w)=0.867 (T=19 degrees C; pH=7.0). Finally, a comparison of model predictions with literature data on growth/no growth responses of S. aureus in culture media and cooked meat was made. Model predictions agreed with published data in 94% of growth cases and in 62% of no growth cases. The latter discordance is highly associated to other environmental factors (such as other preservatives, strains etc.) included in published models that did not match the ones included in our study. This study can help manufacturers in making decision on the most appropriate formulations for food products in order to prevent S. aureus growth and enterotoxin production along their shelf-life.