Dy3+ and Nd3+ induced genetic mutation of bacillus alpha-amylase

Dongyan Zhang; Jianhua Hu; Bater Siqin; Tong Zhang; Ying Chang

doi:10.1016/j.jinorgbio.2008.12.002

Dy3+ and Nd3+ induced genetic mutation of bacillus alpha-amylase

J Inorg Biochem. 2009 Jul;103(7):935-9. doi: 10.1016/j.jinorgbio.2008.12.002. Epub 2008 Dec 24.

Authors

Dongyan Zhang¹, Jianhua Hu, Bater Siqin, Tong Zhang, Ying Chang

Affiliation

¹ School of Chemical Engineering, Department of Biological Engineering, Inner Mongolia University of Technology, Hohhot, China. xym@imut.edu.cn

PMID: 19497621
DOI: 10.1016/j.jinorgbio.2008.12.002

Abstract

After treatment with DyCl(3) and NdCl(3), two strains of mutated bacilli showing increased alpha-amylase activity were isolated. The alpha-amylase genes were amplified, cloned, and sequenced. Sequencing revealed that there were either 11 or 14 bases altered in the two genes. These alterations took the form of base substitutions, and transversion was more common than transition. Based on these results, it was concluded that the rare earth compounds DyCl(3) and NdCl(3) were mutagens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / drug effects*
Bacillus subtilis / enzymology
Bacillus subtilis / genetics
Base Sequence
Cloning, Molecular
Dysprosium / pharmacology*
Molecular Sequence Data
Mutagens / pharmacology*
Mutation
Neodymium / pharmacology*
alpha-Amylases / biosynthesis
alpha-Amylases / genetics*

Substances

Mutagens
Dysprosium
neodymium chloride
Neodymium
alpha-Amylases