Light scattering study of natural DNAs over a wide range of molecular weights: Evidence for compaction of the large molecules

Int J Biol Macromol. 2009 Oct 1;45(3):242-7. doi: 10.1016/j.ijbiomac.2009.05.009. Epub 2009 May 29.

Abstract

This work reports light scattering measurements on DNA in aqueous solutions (100mM NaCl, 1mM EDTA and 10mM Tris-HCl buffer, pH 7.8) over a wide range of molecular weights (10(2)-10(5) base pairs) and shows that, in the above standard solvent, shorter chains (<10(4) base pairs) behave as a "wormlike chain" and their diffusion coefficients as obtained by dynamic light scattering measurements, confirm the prediction of standard wormlike model, whilst longer chains (>10(4) base pairs) behave in a different manner. Dynamic and static light scattering and SEM analysis indicate that DNA molecules 10(5) base pairs long, condense into compact structures in our solvent conditions. Calculations done using a wormlike model are also presented and discussed in comparison both to our experimental data and to other data reported in the literature.

MeSH terms

  • Animals
  • DNA / chemistry*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / ultrastructure
  • Diffusion
  • Humans
  • Light*
  • Micrococcus luteus / chemistry
  • Molecular Weight
  • Scattering, Radiation*

Substances

  • DNA, Bacterial
  • DNA