Carrier PNA for shRNA delivery into cells

Mizuki Kitamatsu; Takanori Kubo; Rino Matsuzaki; Tamaki Endoh; Takashi Ohtsuki; Masahiko Sisido

doi:10.1016/j.bmcl.2009.05.031

Carrier PNA for shRNA delivery into cells

Bioorg Med Chem Lett. 2009 Jul 1;19(13):3410-3. doi: 10.1016/j.bmcl.2009.05.031. Epub 2009 May 15.

Authors

Mizuki Kitamatsu¹, Takanori Kubo, Rino Matsuzaki, Tamaki Endoh, Takashi Ohtsuki, Masahiko Sisido

Affiliation

¹ Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-0082, Japan. kitamatu@cc.okayama-u.ac.jp

PMID: 19481448
DOI: 10.1016/j.bmcl.2009.05.031

Abstract

A peptide nucleic acid (PNA)-cell-penetrating peptide (CPP) conjugate (carrier PNA) was used as 'bridge-builder' to connect a CPP with an shRNA. The carrier PNA successfully formed a hybrid with an shRNA bearing complementary dangling bases and the shRNA was introduced into cells by the carrier PNA, and RNAi was induced by the shRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
CHO Cells
Cricetinae
Cricetulus
Peptide Nucleic Acids / chemistry*
Peptide Nucleic Acids / metabolism
RNA Interference*
RNA, Double-Stranded / administration & dosage*
RNA, Double-Stranded / chemistry
RNA, Double-Stranded / metabolism
Transition Temperature

Substances

Peptide Nucleic Acids
RNA, Double-Stranded