Abstract
In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1d-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Gene Expression Regulation, Plant
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Gene Silencing
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Glutens / genetics
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Glutens / metabolism
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Intramolecular Lyases / genetics
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Intramolecular Lyases / metabolism*
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Oryza / enzymology
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Oryza / genetics*
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Phosphates / metabolism
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Phosphorus / metabolism
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Phytic Acid / biosynthesis*
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / metabolism
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Promoter Regions, Genetic
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Seeds / genetics
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Seeds / growth & development
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Transgenes
Substances
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GluB-1 protein, Oryza
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Phosphates
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Plant Proteins
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Phosphorus
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Phytic Acid
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Glutens
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Intramolecular Lyases
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D-myo-inositol-3-phosphate synthase