Abstract
This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / analysis
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Antioxidants / chemistry*
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Antioxidants / metabolism
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Beverages*
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Biosensing Techniques / methods*
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Cattle
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Citrus sinensis / chemistry*
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Cytochromes c / chemistry*
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Cytochromes c / metabolism
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Electrochemistry
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Electrodes
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Enzymes, Immobilized / chemistry
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Enzymes, Immobilized / metabolism
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Gold / chemistry
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Gold / metabolism
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Horses
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Printing*
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Superoxides / chemistry
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Superoxides / metabolism
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Xanthine Oxidase / chemistry
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Xanthine Oxidase / metabolism
Substances
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Antioxidants
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Enzymes, Immobilized
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Superoxides
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Gold
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Cytochromes c
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Xanthine Oxidase