Rapid PFGE method for fingerprinting of Serratia marcescens isolates

J Microbiol Methods. 2009 Aug;78(2):238-41. doi: 10.1016/j.mimet.2009.05.007. Epub 2009 May 13.

Abstract

We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.

MeSH terms

  • Bacterial Typing Techniques / methods*
  • DNA Fingerprinting / methods*
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field / methods*
  • Genotype
  • Molecular Epidemiology / methods
  • Serratia marcescens / classification*
  • Serratia marcescens / genetics*
  • Time Factors

Substances

  • DNA, Bacterial