Characterization of redox polymer based electrode and electrochemical behavior for DNA detection

Anal Chim Acta. 2009 Jun 8;643(1-2):83-9. doi: 10.1016/j.aca.2009.03.051. Epub 2009 Apr 8.

Abstract

Characterization of redox polymer, poly(vinylferrocenium) perchlorate (PVF+ClO4-) coated as a film on Pt electrodes, and the detection of DNA based on the electrochemical behavior of the polymer were described in this study. PVF+ClO4- modified electrodes were prepared by the electrooxidation of poly(vinylferrocene) (PVF), and DNA immobilized polymer modified electrode was then prepared. The characterization of the polymer modified electrodes were performed by scanning tunneling microscopy (STM), Raman spectroscopy and alternating current (AC) impedance. The effects of DNA concentration, pH, and polymer films in various thicknesses based on the electrode response were also investigated. The electrochemical behavior of DNA modified polymer electrode by using double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) was compared. DNA hybridization was also investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / analysis*
  • Electrochemistry
  • Electrodes
  • Ferrous Compounds / chemistry*
  • Hydrogen-Ion Concentration
  • Membranes, Artificial
  • Microscopy, Scanning Tunneling
  • Oxidation-Reduction
  • Platinum / chemistry*
  • Polyvinyls / chemistry*
  • Spectrum Analysis, Raman

Substances

  • Ferrous Compounds
  • Membranes, Artificial
  • Polyvinyls
  • polyvinylferrocenium
  • Platinum
  • DNA