Objective: To determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.
Method: The separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution: 0-18 min, 26 : 74; 18-33 min, 80 : 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.
Result: The linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).
Conclusion: This method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.