Abstract
Vaccination with the live attenuated Brucella melitensis Rev 1 vaccine is used to control ovine brucellosis caused by Brucella ovis in sheep. The objective of this study was to identify possible correlates of protective response to B. ovis infection through the characterization by microarray hybridization and real-time RT-PCR of inflammatory and immune response genes differentially expressed in rams previously immunized with B. melitensis Rev 1 and experimentally challenged with B. ovis. Gene expression profiles were compared before and after challenge with B. ovis between rams protected and those vaccinated but found infected after challenge. The TLR10, Bak and ANXI genes were expressed at higher levels in vaccinated and protected rams. These genes provide possible correlates of protective response to B. ovis infection in rams immunized with the B. melitensis Rev 1 vaccine.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Annexin A1 / biosynthesis
-
Annexin A1 / genetics
-
Bacterial Vaccines / therapeutic use*
-
Biomarkers / analysis*
-
Biomarkers / metabolism
-
Brucella ovis / immunology*
-
Brucellosis / genetics
-
Brucellosis / immunology
-
Brucellosis / prevention & control
-
Brucellosis / veterinary*
-
Gene Expression
-
Gene Expression Profiling
-
Male
-
RNA / analysis
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sheep
-
Sheep Diseases / genetics*
-
Sheep Diseases / immunology
-
Sheep Diseases / prevention & control*
-
Toll-Like Receptor 10 / biosynthesis
-
Toll-Like Receptor 10 / genetics
-
Vaccines, Attenuated / therapeutic use
-
bcl-2 Homologous Antagonist-Killer Protein / biosynthesis
-
bcl-2 Homologous Antagonist-Killer Protein / genetics
Substances
-
Annexin A1
-
Bacterial Vaccines
-
Biomarkers
-
Rev.1 vaccine, Brucella melitensis
-
Toll-Like Receptor 10
-
Vaccines, Attenuated
-
bcl-2 Homologous Antagonist-Killer Protein
-
RNA