Glycogen synthase kinase-3 (GSK-3) is a multifunctional protein kinase that plays important roles in regulating both glycogen synthesis and protein synthesis. In the present study, we investigated GSK-3beta phosphorylation of silkworm eggs by immunoblotting with a conserved phospho-specific antibody to GSK-3beta. Results showed that the temporal changes in GSK-3beta phosphorylation were closely related to changes in glycogen levels previously reported by other researchers. In diapause eggs, an abrupt decrease in phosphorylation of GSK-3beta was found with the onset of diapause, and phosphorylation level of GSK-3beta reached a minimum level within 1 week after oviposition. However, when diapause eggs were incubated at 25 degrees C for 15 days and then transferred to 5 degrees C, a great increase in GSK-3beta phosphorylation was observed 5 days after transfer to 5 degrees C and high levels were maintained throughout the chilling period. In both non-diapause eggs and eggs whose diapause initiation was prevented by HCl, levels of GSK-3beta phosphorylation appeared to remain relatively high for several days and then greatly decreased 2 or 3 days before hatching. Moreover, GSK-3beta phosphorylation dramatically increased when dechorionated eggs were incubated in medium. The addition of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor, U0126, did not inhibit GSK-3beta phosphorylation in dechorionated eggs, although U0126 dose-dependently inhibited ERK phosphorylation. This result showed that ERK phosphorylation is not involved in upstream signaling for GSK-3beta phosphorylation and that there may be two distinct signaling pathways involved in diapause processing in Bombyx mori eggs.