Abstract
MHC molecules present protein-derived peptides to T lymphocytes. By developing TiO2-based microcentrifugation columns, we identified the first phosphorylated MHC class I ligands from tumor tissue (renal cell carcinoma) and, by comparison to healthy renal tissue, found one Brf1-derived ligand as potentially tumor-associated. We further discovered the first natural phosphorylated MHC class II ligands. They revealed several novel phosphorylation sites of significant transmembrane receptors, such as Frizzled 6, CXCR4 and CD20.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD20 / biosynthesis
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Carcinoma, Renal Cell / metabolism
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Cell Line, Tumor
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Frizzled Receptors / biosynthesis
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Genes, MHC Class I*
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Genes, MHC Class II*
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Histocompatibility Antigens Class I / chemistry*
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Histocompatibility Antigens Class II / chemistry*
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Humans
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Ligands
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Liver Neoplasms / metabolism
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Peptides / chemistry*
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Phosphopeptides / chemistry*
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Phosphorylation
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Receptors, CXCR4 / biosynthesis
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Receptors, G-Protein-Coupled / biosynthesis
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Titanium / chemistry
Substances
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Antigens, CD20
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FZD6 protein, human
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Frizzled Receptors
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Histocompatibility Antigens Class I
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Histocompatibility Antigens Class II
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Ligands
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Peptides
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Phosphopeptides
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Receptors, CXCR4
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Receptors, G-Protein-Coupled
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titanium dioxide
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Titanium