Differential diagnosis of orf viruses by a single-step PCR

J Virol Methods. 2009 Sep;160(1-2):85-9. doi: 10.1016/j.jviromet.2009.04.025. Epub 2009 May 3.

Abstract

The complete nucleotide sequence of the A32L gene (named after vaccinia virus, corresponding with open reading frame 108 of the orf virus and encoding an ATPase) of the orf virus was studied using samples of orf virus from infected goats, which were collected from six outbreaks in central Taiwan. DNA sequence analysis of the A32L genes of these and isolates from other countries showed sequence heterogeneity (base pair variation and deletion) in the 3'-terminal regions. This finding led to the development of a polymerase chain reaction (PCR) method for the rapid differential diagnosis of orf virus infections, and the results demonstrated that this was an easy and reliable method for genotyping of orf viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Animals
  • Base Sequence
  • Diagnosis, Differential
  • Disease Outbreaks
  • Ecthyma, Contagious / diagnosis*
  • Ecthyma, Contagious / epidemiology
  • Ecthyma, Contagious / virology*
  • Genotype
  • Goats
  • Molecular Sequence Data
  • Orf virus / classification*
  • Orf virus / genetics
  • Orf virus / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Taiwan
  • Viral Proteins / genetics

Substances

  • Viral Proteins
  • Adenosine Triphosphatases

Associated data

  • GENBANK/EU935104
  • GENBANK/EU935105
  • GENBANK/EU935106