Despite the increasing level of food hygiene in the Czech Republic and other European Union member states, agents causing diseases transmitted from animals to human remain a serious problem in both human and veterinary medicine of the 21st century. The article is concerned with selected bacterial foodborne zoonoses in the Czech Republic and EU member states, including antibiotic resistance of the pathogens. Some preliminary results on the prevalence of pathogens in the human food chain in the Czech region of Moravia in 2007-2008 are presented.