Biosensing by optical waveguide spectroscopy based on localized surface plasmon resonance of gold nanoparticles used as a probe or as a label

J Colloid Interface Sci. 2009 Jul 1;335(1):140-5. doi: 10.1016/j.jcis.2009.03.016. Epub 2009 Mar 31.

Abstract

The application of localized surface plasmon resonance (LSPR) of gold nanoparticles for the detection of biotin-streptavidin binding, as a typical biological reaction, was investigated by using optical waveguide spectroscopy, and two different modes for the use of gold nanoparticles, one as a probe and the other as a label were compared with each other. The combination with optical waveguide spectroscopy was found to bring about a high sensitivity for the biomolecular detection system using LSPR of gold nanoparticles in both modes. In particular, the mode using gold nanoparticles as a label was demonstrated to be of advantage to devising proper procedures for using nanoparticles and evaluating actual response relevant to the phenomenon concerned, and thus to sensitive detection.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Biotin / metabolism
  • Gold / chemistry*
  • Metal Nanoparticles / chemistry*
  • Protein Binding
  • Sensitivity and Specificity
  • Streptavidin / analysis*
  • Streptavidin / metabolism
  • Surface Plasmon Resonance / instrumentation
  • Surface Plasmon Resonance / methods*

Substances

  • Biotin
  • Gold
  • Streptavidin