In this research, agricultural waste--in particular, comcobs--was investigated for use as the sole carbon source and biofilm carrier to remove nitrate from wastewater in up-flow laboratory reactors. An artificial wastewater with a temperature range of 27 to 33 degrees C was used. Fast startup of the reactor and a high nitrate removal efficiency were observed. The highest denitrification rate of 0.203kg/(m3 x d) was achieved when flow rate and nitrate concentration were 153 L/d and 25.3 mgN/L, respectively. The accumulation of nitrite was not observed in this process. Moreover, flow rate and nitrate concentration of the influent were observed to have a significant effect on nitrate removal efficiency. A sharp decline of nitrate removal efficiency was observed when the flow rate was greater than 50 L/d. The reactor had the ability to accommodate a wide range of pH levels (6.5 to 8.5) and dissolved oxygen (1.5 mg/L to 4 mg/L). A time-dependent decrease in nitrate removal efficiency was observed after 67 days of operation. The addition of fresh corncobs brought about a rapid increase of nitrate removal efficiency. Results showed that corncobs could be used as an economical and effective carbon source for denitrification.