Electron paramagnetic resonance (EPR) spectroscopy and imaging (EPRI) are deeply rooted in the basic and quantum physics, but the spectrum of their applications in modern experimental and clinical dermatology and cosmetology is surprisingly wide. The main aim of this review was to show the physical foundation, technical limitations and versatility of this method in skin studies. Free radical and metal ion detection, EPR dosimetry, melanin study, spin trapping, spin labelling, oximetry and NO-metry, EPR imaging, new generation methods of EPR and EPR/NMR hybrid technology used under ex vivo and in vivo regime are portrayed in the context of clinical and experimental skin research to study problems such as oxidative and nitrosative stress generated by UV or inflammation, skin oxygenation, hydration of corneal layer of epidermis, transport and metabolism of drugs and cosmeceutics, skin carcinogenesis, skin tumors and many others. A part of the paper is devoted to hair and nail research. The review of dermatological applications of EPR is supplemented with a handful of advice concerning practical aspects of EPR experimentation and usage of EPR reagents.